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Rapid and high-throughput analysis of N-glycans from ovarian cancer serum using a 96-well plate platform |
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| Authors: | Yun-Gon Kim Hee-Jin Jeong Yung-Hun Yang Junho Chung |
| Institution: | a Institute of Molecular Biology and Genetics, Seoul National University, Seoul 151-742, Republic of Korea b Institute of Bioengineering, Seoul National University, Seoul 151-742, Republic of Korea c School of Chemical and Biological Engineering in College of Engineering, Seoul National University, Seoul 151-742, Republic of Korea d Department of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul 110-744, Republic of Korea e Department of Biochemistry and Molecular Biology Seoul National University College of Medicine, Seoul 110-744, Republic of Korea |
| Abstract: | We present a rapid and high-throughput human serum N-glycan preparation technology using 96-well plate-based procedures. The released N-glycans from polyvinylidene fluoride (PVDF) membrane filter plate are subsequently loaded to porous graphitic carbon (PGC) containing a 96-well plate to remove salts and other contaminants without sacrificing accuracy or reproducibility. This robust glycan preparation technology is applied to ovarian cancer diagnosis using 5 μl of patient serum. |
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