Efficient fluorography of 3H and 14C on thin layers.

Three methods for efficient detection of ³H and ¹⁴C on thin layers are described. The first method uses 0.4% PPO dissolved in 2-methylnaphthalene. It is approximately 15-fold more efficient than 7% PPO-ether for ³H detection and 15-fold more efficient than autoradiography for ¹⁴C (or ³⁵S) detection. The second method uses PPO dissolved in ether. Efficiency of ³H detection increases as the PPO concentration is increased. The third method uses melted PPO and is as efficient as the first method when the film is exposed at ?78°C.