Quantitative determination of albumin in urine by on-line immunoadsorptive cleanup and reversed-phase chromatography |
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Authors: | E Reh M Kratzer D Zdunek F Lang |
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Institution: | Boehringer-Mannheim GmbH, Department DB-VA, Germany. |
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Abstract: | Albumin in urine is selectively adsorbed on an immunoadsorber (human serum albumin-specific antibodies coupled covalently with a silica stationary phase) and after elution with 0.1% HCl is quantitatively determined by reversed-phase chromatography with detection of native fluorescence. The optimization of sample preparation and characteristics of the method such as recovery, linearity, reproducibility, detection limit, and selectivity are discussed. |
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