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Measurement of DNA length by gel electrophoresis
Authors:E M Southern
Institution:MRC Mammalian Genome Unit, King''s Buildings, West Mains Road, Edinburgh, Scotland
Abstract:Plotting fragment length against reciprocal of mobility gives a straight line over a wider range than the conventional semilogarithmic plot. Curvature can be removed by a simple correction. A method is also given for determining molecular weights from mobilities by direct calculation.
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