A fluorometric assay for dihydrofolate reductase |
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| Authors: | CA Lindquist EC Cadman JR Bertino |
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| Institution: | 1. Department of Medicine, Yale University School of Medicine, New Haven, Connecticut 06510 USA;2. Department of Pharmacology, Yale University School of Medicine, New Haven, Connecticut 06510 USA |
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| Abstract: | A recording fluorometric assay for dihydrofolate reductase is described. The technique measures the appearance of product, tetrahydrofolate, as the reaction proceeds. The assay can be accomplished with as little as 2 pmol of enzyme and is sufficiently sensitive to allow accurate kinetic studies using very low concentrations of substrate. Kinetic studies of both purified and crude preparations of enzyme are reported. |
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