Ultrastructural composition of nonhistone chromosomal skeleton at different stages of mitosis in situ |
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Authors: | M S Makarov Yu S Chentsov |
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Institution: | 1.Moscow State University,Moscow,Russia |
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Abstract: | In interphase cells of the SPEV culture treated with Triton X-100, 2 M NaCl, and DNAse, in the presence of 2 mM CuCl2, we clearly revealed a stabilized nuclear protein material (NPM) composed of a peripheral lamina, residual nucleolus, and
internal fibrillar network. This network is formed by thin fibrils 10–20 nm in diameter, which are also revealed in the nonhistone
matrix of mitotic chromosomes at all stages of mitosis. In mitotic chromosomes, NPM is represented as a network of the 10–20-nm-thick
fibrils without any features of the central-axial structures. Beginning from the middle prophase, it is possible to see approached
sister chromatids in contact with each other in certain sites, similar to centromeres. At these sites, the thickness of fibrils
increases up to 40–50 nm, whereas the fibrils themselves are disposed more tightly; this structure can be seen in the chromosome
until telophase. At the end of telophase, the decondensation of chromosomes and formation of two new nuclei whose NPM is analogous
to NPM of usual interphase nucleus are observed. Thus, the NPM elements can perform the role of a skeleton in both the interphase
nucleus and mitotic chromosomes. |
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