Changes in photosynthetic apparatus of mustard (<Emphasis Type="Italic">Sinapis alba</Emphasis>) cotyledons following cycloheximide and kinetin treatments |
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Authors: | Adriana Machlicová L’udmila Slováková Ján Hudák |
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Institution: | (1) Institute of Botany, Slovak Academy of Sciences, Dúbravská cesta 14, SK-84523 Bratislava, Slovakia;(2) Department of Plant Physiology, Faculty of Natural Sciences, Comenius University in Bratislava, Mlynská dolina B-2, SK-84215 Bratislava 4, Slovakia |
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Abstract: | Biochemical and accompanying structural characteristics of the photosynthetic process were studied in mustard seedlings cultivated
on medium with increasing concentrations of cycloheximide alone as well as in combination with various kinetin concentrations.
After 7 days of cultivation the contents of total chlorophyll, carotenoids and content of Rubisco in mustard cotyledons were
determined. The content of chlorophyll pigments and carotenoids decreased in dependence of cycloheximide concentration. Following
antibiotic treatment the content of both Rubisco subunits markedly decreased. In addition cycloheximide caused disturbance
in mesophyll organization and chloroplast ultrastructure.
Kinetin applied with cycloheximide increased the amount of photosynthetic pigments as well as of Rubisco, compared to the
cycloheximide alone. In the seedlings treated with cycloheximide+kinetin the structure of leaf mesophyll and chloroplast membrane
system was similar to control. Our results indicate that kinetin diminished the negative effects of cycloheximide on photosynthetic
pigments and Rubisco as well as on the structural traits of the cotyledons. |
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Keywords: | Sinapis alba cycloheximide chloroplast ultrastructure kinetin photosynthetic pigments Rubisco |
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