Molecular characterization and expression analysis of a gene encoding mannose-binding lectin from bulb of Zephyranthes grandiflora |
| |
Authors: | Guoyin Kai Yang Lu Zhongying Qian Yongting Luo Genyu Zhou Kexuan Tang |
| |
Institution: | (1) Laboratory of Plant Bioengineering, College of Life and Environment Sciences, Shanghai Normal University, Shanghai, 200234, People’s Republic of China;(2) Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai, 200030, People’s Republic of China |
| |
Abstract: | In this paper we report on the molecular cloning, sequencing and partially characterisation of a lectin from bulb of the Chinese
medicinal plant Zephyranthes grandiflora. The full-length cDNA of Z. grandiflora bulb lectin (ZGBL) consisted of 986 bp and contained a 576 bp ORF encoding a 191 amino acid protein. Bioinformatics analysis
results clearly indicate that ZGBL belongs to the monocot mannose-binding lectin family, which contains 3 putative mannose-binding
sites per subunit. RT-PCR analysis results indicate that ZGBL is constitutively expressed in all the tested tissue types including root, bulb, leaf and flower. Interestingly, ZGBL is
more closely related to the Orchidaceae rather than the Amaryllidaceae family on molecular evolution. |
| |
Keywords: | Zephyranthes grandiflora bulb lectin molecular characterization molecular modelling evolutionary analysis RT-PCR |
本文献已被 SpringerLink 等数据库收录! |
|