Methods for construction of adenovirus vectors |
| |
Authors: | Frank L Graham Ludvik Prevec |
| |
Institution: | (1) Departments of Biology and Pathology, McMaster University, L8S 4K1 Hamilton, Ontario, Canada |
| |
Abstract: | Adenoviruses are attracting increasing attention as general purpose mammalian cell expression vectors, as recombinant vaccines,
and potentially as vectors for gene therapy. Not only is the adenovirus genome relatively easy to manipulate by recombinant
DNA techniques, but adenovirus vectors are relatively stable, grow to high titers, and can transduce a variety of cell types
in cell culture and in vivo. Vectors can be designed that are either replication competent or replication defective and, in
the latter case, are highly efficient at delivering and expressing genes in mammalian cells without resulting in cell killing.
Methods are described for growing, titrating, and purifying adenoviruses, for extracting viral DNA from purified virions and
from infected cells, for rescuing inserts of foreign DNA into the viral genome, and for assessing expression of inserted genes
in adenovirus vectors. |
| |
Keywords: | Adenovirus vectors gene transfer gene therapy vaccines |
本文献已被 SpringerLink 等数据库收录! |
|