Development,Characterization and Cross Species Amplification of Polymorphic Microsatellite Markers from Expressed Sequence Tags of Turmeric (<Emphasis Type="Italic">Curcuma longa</Emphasis> L.) |
| |
Authors: | S Siju K Dhanya S Syamkumar B Sasikumar T E Sheeja A I Bhat V A Parthasarathy |
| |
Institution: | (1) Division of Crop Improvement and Biotechnology, Indian Institute of Spices Research, Calicut, Kerala, 673 012, India;(2) Division of Crop Protection, Indian Institute of Spices Research, Calicut, Kerala, 673 012, India; |
| |
Abstract: | Expressed sequence tags (ESTs) from turmeric (Curcuma longa L.) were used for the screening of type and frequency of Class I (hypervariable) simple sequence repeats (SSRs). A total
of 231 microsatellite repeats were detected from 12,593 EST sequences of turmeric after redundancy elimination. The average
density of Class I SSRs accounts to one SSR per 17.96 kb of EST. Mononucleotides were the most abundant class of microsatellite
repeat in turmeric ESTs followed by trinucleotides. A robust set of 17 polymorphic EST–SSRs were developed and used for evaluating
20 turmeric accessions. The number of alleles detected ranged from 3 to 8 per loci. The developed markers were also evaluated
in 13 related species of C. longa confirming high rate (100%) of cross species transferability. The polymorphic microsatellite markers generated from this
study could be used for genetic diversity analysis and resolving the taxonomic confusion prevailing in the genus. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|