Relatedness of Indian Flax Genotypes (<Emphasis Type="Italic">Linum usitatissimum</Emphasis> L.): An Inter-Simple Sequence Repeat (ISSR) Primer Assay |
| |
Authors: | Ashwini V Rajwade Ritu S Arora Narendra Y Kadoo Abhay M Harsulkar Prakash B Ghorpade Vidya S Gupta |
| |
Institution: | (1) Plant Molecular Biology Group, Division of Biochemical Sciences, National Chemical Laboratory, Dr. Homi Bhabha Road, Pune, 411 008, India;(2) Interactive Research School for Health Affairs, Bharati Vidyapeeth University, Pune, 411043, India;(3) AICRP on Linseed, College of Agriculture, Nagpur, 440 001, India; |
| |
Abstract: | The objective of this study was to analyze the genetic relationships, using PCR-based ISSR markers, among 70 Indian flax (Linum
usitatissimum L.) genotypes actively utilized in flax breeding programs. Twelve ISSR primers were used for the analysis yielding 136 loci,
of which 87 were polymorphic. The average number of amplified loci and the average number of polymorphic loci per primer were
11.3 and 7.25, respectively, while the percent loci polymorphism ranged from 11.1 to 81.8 with an average of 63.9 across all
the genotypes. The range of polymorphism information content scores was 0.03–0.49, with an average of 0.18. A dendrogram was
generated based on the similarity matrix by the Unweighted Pair Group Method with Arithmetic Mean (UPGMA), wherein the flax
genotypes were grouped in five clusters. The Jaccard’s similarity coefficient among the genotypes ranged from 0.60 to 0.97.
When the omega-3 alpha linolenic acid (ALA) contents of the individual genotypes were correlated with the clusters in the
dendrogram, the high ALA containing genotypes were grouped in two clusters. This study identified SLS 50, Ayogi, and Sheetal
to be the most diverse genotypes and suggested their use in breeding programs and for developing mapping populations. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|