Purification and cloning of DNA fragments fractionated on agarose gels |
| |
Authors: | Hugh G Griffin Michael J Gasson |
| |
Institution: | (1) Genetics and Microbiology Department, Institute of Food Research, Norwich Research Park, NR4 7UA Colney, Norwich, UK |
| |
Abstract: | Purification of DNA fragments from acrylamide or agarose gels is a commonly used technique in the molecular biology laboratory.
This article describes a rapid, efficient, and inexpensive method of purifying DNA fractions from an agarose gel. The purified
DNA is suitable for use in a wide range of applications including ligation using DNA ligase. The procedure uses standard high-melting-temperature
agarose and normal TBE electrophoresis buffer. In addition, the protocol does not involve the use of highly toxic organic
solvents such as phenol. |
| |
Keywords: | Agarose gel DNA purification DNA fractionation ligation electrophoresis molecular biology |
本文献已被 SpringerLink 等数据库收录! |