Molecular Characterization of <Emphasis Type="Italic">Kluyveromyces marxianus</Emphasis> Strains Isolated from <Emphasis Type="Italic">Agave fourcroydes</Emphasis> (Lem.) in Yucatan,Mexico |
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| Authors: | Daisy Pérez-Brito Raúl Tapia-Tussell Andrés Quijano-Ramayo Alfonso Larqué-Saavedra Patricia Lappe |
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| Institution: | (1) Laboratorio GeMBio, Centro de Investigación Científica de Yucatán, Calle 43 # 130, Col. Chuburná de Hidalgo, Merida, 97200, Yucatan, Mexico;(2) Instituto de Biología, Universidad Nacional Autónoma de México, 3er Circuito S/N, Ciudad Universitaria, Del. Coyoacán, Mexico, DF, 04510, Mexico |
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| Abstract: | The molecular characterization of 14 strains of Kluyveromyces marxianus isolated from Agave fourcroydes (Lem.) in Yucatan, Mexico, was performed by AP-PCR analysis, PCR-RFLP of 5.8S-ITS, and complete NTS regions. A sequence analysis
of the D1/D2 domain of the 26S rDNA was also carried out in six selected strains. The AP-PCR approach had the highest discrimination
power for the molecular characterization of new henequen K. marxianus strains. PCR-RFLP of 5.8S-ITS regions did not reveal polymorphisms in this group of strains. The restriction enzyme digestion
analysis of NTS region enables the separation among strains which coincides with ascospore shape groups. The molecular tools
used in this article may be useful to confirm a preliminary screen of yeasts isolated from henequen without the use of growth
characteristics or morpho-physiological tests. |
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| Keywords: | Agave fourcroydes Kluyveromyces marxianus AP-PCR analysis PCR-RFLP analysis of rDNA Fingerprinting |
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