De Novo synthesis of PCR templates for the development of SARS diagnostic assay |
| |
| Authors: | Jia Zhang Bo Meng Duanfang Liao Lvyi Zhou Xu Zhang Linling Chen Zifen Guo Cuiying Peng Bingyang Zhu Peggy P Lee Xiangmin Xu Tianhong Zhou Zemin Deng Yinghe Hu Kai Li |
| |
| Institution: | (1) Genomapping Inc., Tianjin;(2) Shanghai Institute of Brain Functional Genomics, East China Normal University, Shanghai;(3) Institute of SNP, Institute of Pharmacy and Pharmacology, National University, Hengyang;(4) University of California in San Diego, San Diego, USA;(5) Department of Medical Genetics, The First Military Medical University, Guangzhou;(6) Institute of Life Science and Biotechnology, Jinana University, Guangzhou;(7) Genomics Group, Loma Linda, University, CA |
| |
| Abstract: | A novel coronavirus was identified as the cause for severe acute respiratory syndrome (SARS). The complete sequence of SARS
genome has provided an opportunity for the development of molecular diagnostic assays. To restrain further outbreak of SARS,
the World Health Organization has posted several pairs of polymerase chain reaction (PCR) primers for early diagnosis and
urged more research to be done on PCR protocols. Here we report a strategy for the de novo synthesis of PCR templates complimentary to the SARS virus genome, which has the advantage of working on PCR templates without
concern about viral infection and also has the advantage that it can be used by those who do not have access to the SARS virus.
This highly efficient and safe strategy for obtaining SARS gene fragments is useful for the development of PCR assays, as
well as for the preparation of reliable positive controls for PCR testing kits. |
| |
| Keywords: | Gene synthesis SARS PCR sequential primer extension |
| 本文献已被 PubMed SpringerLink 等数据库收录！ |
|
