Cell adhesion assays |
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Authors: | Martin J Humphries |
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Institution: | (1) School of Biological Sciences, University of Manchester, 2.205 Stopford Building, Oxford Road, M13 9PT Manchester |
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Abstract: | Cell adhesion makes an important contribution to the maintenance of tissue structure, the promotion of cell migration, and
the transduction of information about the cell microenvironment across the plasma membrane. An ability to quantitate adhesion
has proven to be extremely valuable for those researchers studying the molecular mechanisms underlying these processes. This
article will outline in detail two standard assays used for quantitating the adhesion of cells to an immobilized substrate.
First, an attachment assay, which employs a colorimetric detection of bound cells, and second, a spreading assay, which employs
phase contrast microscopy to measure the flattening of adherent cells. |
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Keywords: | Adhesion integrin spreading attachment extracellular matrix |
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