Effect of the level of ATP and of the state of spectrin on osmotic properties of bovine erythrocytes |
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Authors: | M Mosior M Bobrowska J Gomu?kiewicz |
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Institution: | Institute of Physics, Technical University of Wroc?aw, Poland. |
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Abstract: | The effect of the intracellular level of ATP and of the state of spectrin on the critical cell volume of bovine erythrocyte was studied. The state of spectrin was changed by thermal denaturation, which for the bovine red cell took place at similar temperature as for the human erythrocyte. The increase of the ATP level and the spectrin denaturation increased the critical cell volume, while metabolic starvation decreased it. The changes of the ATP level did not influence the critical volume after the denaturation of spectrin. The results suggest that the ATP-dependent effect on the critical cell volume was caused by an alteration of the membrane extensibility due to the change of the membrane skeleton-lipid bilayer interaction(s). |
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