Cloning and DNA sequence analysis of a polygalacturonase cDNA from Aspergillus niger RH5344 |
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| Authors: | E Ruttkowski R Labitzke N Q Khanh F L?ffler M Gottschalk K D Jany |
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| Institution: | Institut für Biochemie, TH Darmstadt, F.R.G. |
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| Abstract: | A 1319 bp long cDNA encoding for a polygalacturonase (EC 3.2.1.15) from Aspergillus niger RH5344 comprises a single open reading frame of 1089 bp which includes the mature protein of 362 amino acids and an NH2-terminal signal peptide of 27 amino acids. The directly determined peptides of the mature polygalacturonase confirmed the sequence information deduced from the cDNA. |
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