Lymphocyte subset analysis for the assessment of treatment-related complications after autologous stem cell transplantation in multiple myeloma |
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| Authors: | Lee Sung-Eun Yahng Seung-Ah Cho Byung-Sik Eom Ki-Seong Kim Yoo-Jin Kim Hee-Je Lee Seok Cho Seok-Goo Kim Dong-Wook Lee Jong-Wook Min Woo-Sung Park Chong-Won Min Chang-Ki |
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| Institution: | 1. Department of Haematology, University College London, London, UK;2. Department of Haematology, University College London Hospitals Trust, London, UK;3. Clinical Research Informatics, International Clinical Research Center Research Institute, Tokyo, Japan;4. Clinical Laboratory and Pathology, National Center for Global Health and Medicine, Tokyo, Japan;5. Division of AIDS Clinical Center, National Center for Global Health and Medicine, Tokyo, Japan;1. Gastroenterology and Liver Services, Sydney Local Health District, Concord Hospital, Sydney, Australia;2. Faculty of Medicine, The University of New South Wales, Sydney, Australia;3. Sydney Medical School, University of Sydney, Sydney, Australia;1. Helmholtz-Institute for Biomedical Engineering, RWTH Aachen University Medical School, Aachen, Germany;2. Interdisciplinary Center of Scientific Computing (IWR), Institute of Applied Mathematics, University of Heidelberg, Heidelberg, Germany;3. Heidelberg Academy of Sciences and Humanities, Heidelberg, Germany;4. Department of Medicine V, University of Heidelberg, Heidelberg, Germany;5. Department of Plastic and Reconstructive Surgery, Hand Surgery, Burn Center, RWTH Aachen University Medical School, Aachen, Germany;1. Thomas E. Starzl Transplantation Institute, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, USA;2. Revivicor Inc., Blacksburg, Virginia, USA;1. Research Center for Cancer Stem Cell, Tokai University School of Medicine, Isehara, Kanagawa, Japan;2. Department of Hematology/Oncology, Tokai University School of Medicine, Isehara, Kanagawa, Japan;3. Department of Laboratory Medicine, Tokai University School of Medicine, Isehara, Kanagawa, Japan;4. Support Center for Medical Research and Education, Tokai University School of Medicine, Isehara, Kanagawa, Japan;5. Medical Research Institute, Tokai University, Isehara, Kanagawa, Japan |
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| Abstract: | Background aimsThe aim of this study was to investigate the correlation between infused lymphocyte populations and lymphocyte subsets at engraftment, and the early clinical implications of lymphocyte subset recovery after autologous stem cell transplantation (ASCT) in multiple myeloma (MM).MethodsWe examined the lymphocyte populations of infused autografts and the lymphocyte subsets of peripheral blood at engraftment from 50 patients using flow cytometry. Each subset was grouped as low (below median) and high (above median) to examine the correlation with mucositis of grade 3 or more and the occurrence of infections and cytomegalovirus (CMV) reactivation.ResultsUsing Spearman correlation coefficients, we found that cell doses of infused CD8+ (P = 0.042) and CD19+ cells (P = 0.044) were significantly associated with the absolute lymphocyte count (ALC) at engraftment. The dose of infused CD34+ cells was not associated with the change of lymphocyte subsets except for an inverse correlation with CD4+ cells (P = 0.006). After adjusting for potential variables in univariate analysis, multivariate analyzes revealed that the lower ratio of infused CD4+ to CD8+ cells (P = 0.030) was an independent factor for severe mucositis. Of lymphocyte subsets at engraftment, a higher frequency of CD3+ (P = 0.024) and a lower frequency of CD56+ (P = 0.020) were independent predictors for infections after engraftment. A higher frequency of CD8+ cells (P = 0.041) and a lower ratio of CD4+ to CD8+ (P = 0.021) were independent predictors for CMV reactivation.ConclusionsOur data suggest that lymphocyte subset analysis of infused autograft and peripheral blood at engraftment may provide new predictors for early complications after ASCT in patient with MM. |
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