Autologous rabbit adipose tissue-derived mesenchymal stromal cells for the treatment of bone injuries with distraction osteogenesis |
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| Authors: | Ozgur Sunay Geylani Can Zeynep Cakir Ziya Denek Ilknur Kozanoglu Guven Erbil Mustafa Yilmaz Yusuf Baran |
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| Institution: | 1. Department of Plastic Reconstructive and Aesthetic Surgery, Dokuz Eylul University, School of Medicine, Balcova, Izmir, Turkey;2. Department of Histology and Embryology, Dokuz Eylul University, School of Medicine, Balcova, Izmir, Turkey;3. Department of Molecular Biology and Genetics, Izmir Institute of Technology, Faculty of Science, Urla, Izmir, Turkey;4. Adana Teaching and Medical Research Center, Bone Marrow Transplantation Unit, Baskent University, School of Medicine, Adana, Turkey;1. Department of Child Neurology, National Center of Neurology and Psychiatry (NCNP), Kodaira, Tokyo, Japan;2. Department of Neurosurgery, NCNP, Kodaira, Tokyo, Japan;3. Department of Psychiatry, NCNP, Kodaira, Tokyo, Japan;4. Department of Pain Clinic, NTT Medical Center Tokyo, Shinagawa, Tokyo, Japan;1. Department of Cardiology, the First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, Guangdong Province, China;2. Department of Cardiology, the Affiliated Hospital at Shunde (the First People''s Hospital of Shunde), Southern Medical University, Foshan, Guangdong Province, China;1. Dokuz Eylul University, Faculty of Medicine, Department of Orthopaedics and Traumatology, 35340 Balcova, Izmir, Turkey;2. Gazikent Medical Center, Department of Orthopaedics and Traumatology, 35340 Gazi Emir, Izmir, Turkey;3. ?ifa University Faculty of Medicine, Orthopedics and Traumotology Department, Sanayi St. No: 7, 35040 Bornova, Izmir, Turkey;4. Dokuz Eylul University, Health Science Institute, Department of Biomechanics, 35340 Balcova, Izmir, Turkey;1. Service de rhumatologie, Hôtel-Dieu, CHU de Nantes, 44093 Nantes cedex 01, France;2. Service de rhumatologie, CHU de Reims, 45, rue Cognacq-Jay, 51092 Reims, France |
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| Abstract: | Background aimsAdipose tissue-derived mesenchymal stromal cells (MSCs) have a higher capacity for proliferation and differentiation compared with other cell lineages. Although distraction osteogenesis is the most important therapy for treating bone defects, this treatment is restricted in many situations. The aim of this study was to examine the therapeutic potential of adipose tissue-derived MSCs and osteoblasts differentiated from adipose tissue-derived MSCs in the treatment of bone defects.MethodsBone defects were produced in the tibias of New Zealand rabbits that had previously undergone adipose tissue extraction. Tibial osteotomy was performed, and a distractor was placed on the right leg of the rabbits. The rabbits were placed in control (group I), stem cell (group II) and osteoblast-differentiated stem cell (group III) treatment groups. The rabbits were sacrificed, and the defect area was evaluated by radiologic, biomechanical and histopathologic tests to examine the therapeutic effects of adipose tissue-derived MSCs.ResultsRadiologic analyses revealed that callus density and the ossification rate increased in group III compared with group I and group II. In biomechanical tests, the highest ossification rate was observed in group III. Histopathologic studies showed that the quality of newly formed bone and the number of cells active in bone formation were significantly higher in group III rabbits compared with group I and group II rabbits.ConclusionsThese data reveal that osteoblasts differentiated from adipose tissue-derived MSCs shorten the consolidation period of distraction osteogenesis. Stem cells could be used as an effective treatment for bone defects. |
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