Choosing the right type of serum for different applications of human adipose tissue–derived stem cells: influence on proliferation and differentiation abilities |
| |
| Institution: | 1. Clinic for Plastic and Reconstructive Surgery, Aesthetic and Preventive Medicine at Heidelberg University Hospital–ETHIANUM, Heidelberg, Germany;2. Department of Cardiology, University of Frankfurt, Frankfurt, Germany;1. Department of Pediatric Hematology/Oncology, IRCCS Bambino Gesù Children''s Hospital, Rome, Italy;2. University of Pavia, Pavia, Italy;3. Department of Biomedical, Surgical, Dental Sciences, University of Milan, Italy;4. UCV Neurobiology, IRCCS Neurological Institute, Carlo Besta, Milan, Italy;1. Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran;2. Department of Cell Engineering, Cell Science Research Center, Royan Institute, ACECR, Tehran, Iran;3. Department of Stem Cells and Developmental Biology, Cell Sciences Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran;4. Department of Developmental Biology, University of Science and Culture, Tehran, Iran;1. Human Metabolomics, North-West University, Private Bag X6001, Potchefstroom, 2520, South Africa;2. Centre of Excellence for Pharmaceutical Sciences, North-West University, Private Bag X6001, Potchefstroom, 2520, South Africa;3. Department of Biochemistry and Molecular Biology, University of Southern Denmark, DK-5230 Odense, Denmark;1. Institute of Life Science, College of Medicine, Swansea University, United Kingdom;2. Centre for Nanotechnology & Regenerative Medicine, University College London, United Kingdom;1. Heart Failure Program, Division of Cardiovascular Medicine, University of Massachusetts Medical Center, Worcester, Massachusetts;2. Dynabio S.A., Marseille, France |
| |
| Abstract: | Background aimsAdipose tissue–derived stem cells (ADSCs) are thought to have great potential in regenerative medicine. A xenoprotein-free culture and handling system is desirable. To date, there is only little and contradictory information about the influence of the different types of human serum on ADSC proliferation and differentiation.MethodsFirst, ADSCs were cultured in media containing regular human serum (HS plus) or fetal calf serum (FCS plus) with supplementation of growth factors for three passages. During passage 4, ADSC proliferative activity and adipogenic, osteogenic and chondrogenic differentiation ability was quantified. Second, ADSCs were cultured with three different human sera (regular human serum HS], human serum from platelet-poor plasma SPPP] or human serum from platelet-rich plasma SPRP]) without supplementation of platelet-derived growth factor and assessed accordingly. The growth factor content of the different types of human sera was determined by means of multiplex protein assay and enzyme-linked immunosorbent assay.ResultsThe different sera did not affect ADSC doubling time significantly (P < 0.05). Specific glycerol-3-phosphat-dehydrogenase activity was significantly lower in cultures with SPRP (P < 0.01) compared with the other media compositions. Extracellular calcium deposition was significantly higher in cells differentiated in cultures with HS or SPPP compared with those with SPRP, HS plus or FCS (P < 0.01). Glycosaminoglycan content and collagen 2 were highest in cells cultured with SPRP (P < 0.001).ConclusionsCulturing ADSCs in human serum appears to be a reasonable and efficient alternative compared with FCS. With respect to the outcome of a sighted clinical application, it appears to be feasible to handle the cells in a serum suitable for the intended later use. |
| |
| Keywords: | adipose tissue differentiation human serum platelet-derived growth factor proliferation stem cells |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
