Co-transplantation of olfactory ensheathing glia and mesenchymal stromal cells does not have synergistic effects after spinal cord injury in the rat |
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| Authors: | Takashi Amemori Pavla Jendelová Kate?ina R??i?ková David Arboleda Eva Syková |
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| Institution: | 1. Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic;2. Department of Neuroscience and Center for Cell Therapy and Tissue Repair, Charles University, 2nd Medical Faculty, Prague, Czech Republic;1. Field Neurosciences Institute Laboratory for Restorative Neurology, Central Michigan University, Mount Pleasant, MI 48859, USA;2. Program in Neurosciences, Central Michigan University, Mount Pleasant, MI 48859, USA;3. Department of Psychology, Central Michigan University, Mount Pleasant, MI 48859, USA;4. College of Medicine, Central Michigan University, Mount Pleasant, MI 48859, USA;5. Field Neurosciences Institute, 4677 Towne Centre Rd. Suite 101, Saginaw, MI 48604, USA;1. Department of Otolaryngology Head & Neck Surgery, Beijing Chaoyang Hospital, Key Laboratory of Otolaryngology Head and Neck Surgery, Capital Medical University, Beijing, PR, China;2. Clinic of Beijing Branch, China Southern Airlines Co., Ltd., PR, China;1. School of Medicine, Jeju National University, Jeju 690-756, Republic of Korea;2. Department of Veterinary Anatomy, College of Veterinary Medicine, Chonnam National University, Gwangju 500-757, Republic of Korea;3. College of Veterinary Medicine, Jeju National University, Jeju 690-756, Republic of Korea;4. Departments of Neurosurgery, College of Medicine, Jeju National University, Jeju 690-756, Republic of Korea;1. Department of Neurology, Research Institute of Neuromuscular and Neurodegenerative Diseases, Qilu Hospital of Shandong University, Jinan, 250012, China;2. Department of Neurosurgery, Qilu Hospital of Shandong University and Institute of Brain and Brain-Inspired Science, Shandong University, Jinan, 250012, China;1. Division of Neurochemistry and Neuropathology, Department of Neurology, Poznan University of Medical Sciences (PUMS), 49 Przybyszewskiego Street, 60-355 Poznan, Poland;2. Department of Neurology and Cerebrovascular Disorders, Poznan University of Medical Sciences, 34 Dojazd Street, 60-631 Poznan, Poland;3. Department of Neurology, Poznan University of Medical Sciences, 49 Przybyszewskiego Street, 60-355 Poznan, Poland |
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| Abstract: | Background aimsOlfactory ensheathing glia (OEG) and mesenchymal stromal cells (MSC) are suitable candidates for transplantation therapy of spinal cord injury (SCI). Both facilitate functional improvement after SCI by producing trophic factors and cytokines. In this study, the co-transplantation of both types of cells was studied to clarify their additive and/ or synergistic effects on SCI.MethodsA balloon-induced compression lesion was used to produce SCI in rats. OEG, MSC or both OEG and MSC (3 × 105 cells of each cell type) were implanted by intraspinal injection 1 week after SCI. The effect of transplantation was assessed using behavioral, electrophysiologic and histologic methods.ResultsHindlimb function was examined with Basso, Beattie and Bresnahan (BBB) and Plantar tests. Improvement was found in all three groups of transplanted rats with different time–courses, but there was no significant difference among the groups at the end of the experiment. Motor-evoked potentials after SCI decreased in amplitude from 7 mV to 10 µV. Linear regression analysis showed a modest recovery in amplitude following transplantation, but no change in the control rats. Histologic findings showed that the white and gray matter were significantly spared by transplantation after SCI.ConclusionsFunctional improvement was achieved with transplantation of OEG and/or MSC, but the co-transplantation of OEG and MSC did not show synergistic effects. The poor migration of OEG and MSC might prevent their concerted action. Pre-treatment with a Rho antagonist and a combination of intraspinal and intravenous injection of the cells might be beneficial for SCI therapy. |
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