Transcranial low-level laser therapy in an in vivo model of stroke: Relevance to the brain infarct,microglia activation and neuroinflammation |
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| Authors: | Débora D S Vogel Nancy N Ortiz-Villatoro Noemi S Araújo Márcia Jonathas Guimarães Marques Flavio Aimbire Fúlvio A Scorza Carla A Scorza Regiane Albertini |
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| Institution: | 1. Disciplina de Neurociência, Departamento de Neurologia e Neurocirurgia, Escola Paulista de Medicina (EPM), Universidade Federal de São Paulo (UNIFESP), São Paulo, Brazil;2. Programa de Pós-graduação em Medicina Translacional, Departamento de Ciência e Tecnologia, Universidade Federal de São Paulo (UNIFESP), São Paulo, Brazil;3. Programa de Pós-graduação em Ciência do Movimento Humano e Reabilitação, Departamento de Ciência e Tecnologia, Universidade Federal de São Paulo (UNIFESP), São Paulo, Brazil |
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| Abstract: | Stroke is the main cause of death and functional disability. The available therapy affects only 5% of patients, and new therapeutic approaches have been constantly tested. Transcranial photobiomodulation (PBM) is promising for its neuroprotective effect on brain injuries. Thus, the present study investigated the PBM effects in an in vivo model of ischemic stroke induced by photothrombosis (PT). Five different groups of Wistar rats were submitted or not to a daily dose of fish oil or/and laser sessions for 2 months. The ischemia volume was evaluated by stereology; GFAP, Iba and NeuN by immunohistochemistry; TNF-α, IL-1β, IL-6, IL-10 and TGF-β by ELISA assay. PBM influenced both the lesion volume and the GFAP. Furthermore, PBM and Ω-3 or both reduced Iba RNAm. PBM reduced TNF-α, IL-1β, IL-6, brain damage, neuroinflammation and microglial activation, and it increased astroglial activity in peri-lesioned region after stroke. |
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| Keywords: | brain infarct neuroinflammation omega-3 photobiomodulation stroke |
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