Structured illumination microscopy with interleaved reconstruction (SIMILR) |
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| Authors: | Ying Ma Di Li Zachary J Smith Dong Li Kaiqin Chu |
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| Institution: | 1. Precision Machinery & Precision Instrumentation, University of Science and Technology of China, Hefei, China;2. National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China |
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| Abstract: | Structured illumination microscopy (SIM) is the commonly used super‐resolution (SR) technique for imaging subcellular dynamics. However, due to its need for multiple illumination patterns, the frame rate is just a fraction of that of conventional microscopy and is thus too slow for fast dynamic studies. A new SR image reconstruction method that maximizes the use of each subframe of the acquisition series is proposed for improving the super‐resolved frame rate by N times for N illumination directions. The method requires no changes in raw data and is appropriate for many versions of SIM setup, including those implementing fast illumination pattern generation mechanism based on spatial light modulator or digital micromirror device. The performance of the proposed method is demonstrated through imaging the highly dynamic endoplasmic reticulum where continuous rapid growths or shape changes of tiny structures are observed.  |
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| Keywords: | data processing high‐speed imaging image reconstruction structured illumination microscopy |
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