Isolation of Actinomycetes from Soil Using Extremely High Frequency Radiation |
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Authors: | Li Yu V Terekhova L P Gapochka M G |
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Institution: | (1) Research Institute of New Antibiotics, Russian Academy of Medical Sciences, ul. Bol'shaya Pirogovskaya 11, Moscow, 119867, Russia;(2) Faculty of Physics, Moscow State University, Vorob'evy gory, Moscow, 119899, Russia |
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Abstract: | A new method employing extremely high frequencies (EHFs) is proposed for the selective isolation of actinomycetes from soil. The pretreatment of soil suspensions with EHF wavelengths of 5.6 and 7.1 mm led to a nonselective isolation of actinomycetes. At the same time, the irradiation of soil suspensions within wavelength bands of 3.8–5.8 and 8–11.5 mm considerably augmented the total number of isolated actinomycetes and increased the fraction of the isolated rare genera by 2 and 7 times, respectively. The rare actinomycete genera were represented by Actinomadura, Microtetraspora, Nonomuraea, Micromonospora, Amycolatopsis, Pseudonocardia, Saccharotrix, and Streptosporangium. |
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Keywords: | actinomycetes rare genera of actinomycetes EHF radiation |
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