<Emphasis Type="Italic">Anoxybacillus mongoliensis</Emphasis> sp. nov., a novel thermophilic proteinase producing bacterium isolated from alkaline hot spring,Central Mongolia |
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Authors: | Z B Namsaraev O B Babasanova Y E Dunaevsky V N Akimov D D Barkhutova V M Gorlenko B B Namsaraev |
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Institution: | 1.Winogradsky Institute of Microbiology RAS,Moscow,Russia;2.Institute of General and Experimental Biology Siberian Branch RAS,Ulan-Ude,Russia;3.Belozersky Institute of Physico-Chemical Biology,Moscow State University,Moscow,Russia;4.Skryabin Institute of Biochemistry and Physiology of Microorganisms RAS,Pushchino, Moscow oblast,Russia;5.Buryat State University,Ulan-Ude,Russia |
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Abstract: | A Gram reaction positive, spore-forming, facultative anaerobic bacterium belonging to the Phylum Firmicutes, was isolated from alkaline hot (80°C, pH 9.8) spring Tsenher, Central Mongolia. The cells were rod shaped, feebly motile,
peritrichously flagellated. Strain T4T was moderately thermophilic with optimum growth at 60°C. Maximum temperature for growth
was between 70 and 75°C; minimum temperature for growth was between 35 and 30°C. Alkalitolerant, optimum pH for growth was
8.0; minimum pH for growth was between 5.0 and 5.5 and maximum was between 10.5 and 10.8. The growth was observed at NaCl
concentrations of 0–5% (w/v) with the optimum at 0.2–0.5%. No growth was observed at 6% NaCl (w/v). Aerobically, the strain
utilized proteinaceous substrates, organic acids and a range of carbohydrates including glucose, ribose, sucrose and xylose
as well. Anaerobically, only glucose and sucrose were utilized. Strain T4T produced thermostable alkaline subtilisin-like serine proteinase. The G + C content was 44.2 mol % (td). On the basis of
16S rRNA gene sequence similarity strain T4T was shown to be closely related to the members of the genus Anoxybacillus (family Bacillaceae, class “Bacilli”). DNA-DNA hybridization data revealed that strain T4T had only 38% relatedness to A. flavithermus and 28% relatedness to A. pushchinoensis. Based on its morphology, physiology, phylogenetic relationship and its low DNA-DNA relatedness values with validly published
species of Anoxybacillus, it is proposed that strain T4T represents a novel species Anoxybacillus mongoliensis sp. nov., with the type strain T4T (=DSM 19169 =VKM 2407). |
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