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Genotyping of <Emphasis Type="Italic">Aeromonas hydrophila</Emphasis> by Box elements
Authors:Vijai Singh  Dharmendra Kumar Chaudhary  Indra Mani  Pallavi Somvanshi  Gaurav Rathore  Neeraj Sood
Institution:(1) Aquatic Pathology Laboratory, National Institute of Oceanography and Fisheries, Cairo, Egypt
Abstract:PCR-based DNA fingerprinting techniques were evaluated to genotype eight diseased, particularly normal and environmental isolates of Aeromonas hydrophila. PCR-based fingerprinting method has an advantage of having repetitive sequence also called Box elements that are interspersed throughout the genome in diverse bacterial species. The BOX-PCR fingerprinting technique was evaluated for the discrimination of different isolates of A. hydrophila. All the studied isolates have shown major banding patterns ranged from 500–3000 bp. These finding could be advantageous to investigate the strain level specific fingerprints of A. hydrophila as potential genotypic markers.
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