Genotyping of <Emphasis Type="Italic">Aeromonas hydrophila</Emphasis> by Box elements |
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Authors: | Vijai Singh Dharmendra Kumar Chaudhary Indra Mani Pallavi Somvanshi Gaurav Rathore Neeraj Sood |
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Institution: | (1) Aquatic Pathology Laboratory, National Institute of Oceanography and Fisheries, Cairo, Egypt |
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Abstract: | PCR-based DNA fingerprinting techniques were evaluated to genotype eight diseased, particularly normal and environmental isolates
of Aeromonas hydrophila. PCR-based fingerprinting method has an advantage of having repetitive sequence also called Box elements that are interspersed
throughout the genome in diverse bacterial species. The BOX-PCR fingerprinting technique was evaluated for the discrimination
of different isolates of A. hydrophila. All the studied isolates have shown major banding patterns ranged from 500–3000 bp. These finding could be advantageous
to investigate the strain level specific fingerprints of A. hydrophila as potential genotypic markers. |
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