Role of the Polysaccharide Components of Azospirillum brasilense Capsules in Bacterial Adsorption on Wheat Seedling Roots |
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Authors: | Egorenkova I V Konnova S A Fedonenko Yu P Dykman L A Ignatov V V |
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Institution: | (1) Laboratory of Biochemistry, Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 13 Prosp. Entuziastov, 410049 Saratov, Russia;(2) Laboratory of Chemistry, Department of Science, Agricultural University of Athens, Iera Odos 75, 11855 Athens, Greece |
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Abstract: | Azospirillum brasilense cells deprived of capsular exopolysaccharides completely lost their ability to bind wheat germ agglutinin (WGA) and much of their ability to attach to wheat seedling roots. The decapsulation of bacterial cells by washing them with a NaCl solution led to an increase in the relative hydrophobicity of the cell surface. The pretreatment of wheat seedling roots with N-acetyl-D-glucosamine (GlcNAc) or the GlcNAc-containing polysaccharide complexes stripped from Azospirillum cells reduced their attachment to the roots. Under the experimental conditions used (3-h incubation of wheat seedling roots with exponential-phase azospirilla), bacterial adsorption is mainly driven by the specific mechanisms attachment of the cells to the roots, whose operation is due to the capsular polysaccharide components and the WGA present on the wheat seedling roots. |
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Keywords: | Azospirillum brasilense capsule extracellular polysaccharide adsorption wheat |
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