Introduction of antioxidant-loaded liposomes into endothelial cell surfaces through DNA hybridization |
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| Institution: | 1. Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of education, Shaanxi Key Laboratory of Physico-Inorganic Chemistry, College of Chemistry & Material Science, Northwest University, Xi''an, Shaanxi 710069, PR China;2. Department of Chemistry, University of Malaya, Kuala Lumpur 50603, Malaysia;3. Chemistry Department, King Abdulaziz University, Jeddah 80203, Saudi Arabia;4. Department of Geology, Northwest University, Xi''an 710069, PR China |
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| Abstract: | Ischemia–reperfusion damage is a problem in organ transplantation. Reactive oxygen species are produced in cells by blood-mediated reactions at the time of blood reperfusion. In this study, we developed a method to immobilize and internalize antioxidants in endothelial cells, using vitamin E-loaded liposomes. The liposomes loaded with vitamin E and human umbilical vein endothelial cells (HUVECs) were modified with poly(ethylene glycol)–phospholipid conjugates carrying 20-mer of deoxyadenylic acid (oligo(dA)20) and 20-mer of complementary deoxythymidylic acid (oligo(dT)20), respectively. The liposomes were effectively immobilized on HUVECs through DNA hybridization between oligo(dA)20 and oligo(dT)20. The liposomes loaded with vitamin E were gradually internalized into HUVECs. Then, the cells were treated with antimycin A to induce oxidative stress. We found the amount of reactive oxygen species was greatly reduced in HUVECs carrying vitamin E-loaded liposomes. |
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| Keywords: | Ischemia Reperfusion injury Endothelial cell Surface modification Vitamin E Liposome Poly(ethylene glycol)–phospholipid DNA hybridization |
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