Chemoenzymatic synthesis of ADP-d-glycero-β-d-manno-heptose and study of the substrate specificity of HldE |
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| Institution: | 1. College of Pharmacy, Nankai University, Weijin Road 94, Nankai District, Tianjin, 300071, People''s Republic of China;2. State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, Nankai University, Weijin Road 94, Nankai District, Tianjin, 300071, People''s Republic of China |
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| Abstract: | An efficient one-pot three enzymes strategy for chemoenzymatic synthesis of ADP-d-glycero-β-d-manno-heptose (ADP-d, d-heptose) was reported using chemically synthesized d, d-heptose-7-phosphate and the ADP-d, d-heptose biosynthetic enzymes HldE and GmhB. Moreover, the result of investigating substrate specificity of the kinase action of HldE revealed that HldE had highly restricted substrate specificity towards structurally modified heptose-7-phosphate analogs. |
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| Keywords: | Chemoenzymatic Synthesis Analogs Specificity |
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