Calmodulin inhibitors from the fungus Emericella sp. |
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Authors: | Mario Figueroa María del Carmen González Rogelio Rodríguez-Sotres Alejandro Sosa-Peinado Martín González-Andrade Carlos M Cerda-García-Rojas Rachel Mata |
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Institution: | 1. Facultad de Química, Universidad Nacional Autónoma de México, México DF 04510, Mexico;2. Instituto de Biología, Universidad Nacional Autónoma de México, México DF 04510, Mexico;3. Facultad de Medicina, Universidad Nacional Autónoma de México, México DF 04510, Mexico;4. Departamento de Química, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, México DF 07000, Mexico |
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Abstract: | Two new xanthones identified as 15-chlorotajixanthone hydrate (1) and 14-methoxytajixanthone (2) were isolated from an Emericella sp. strain 25379 along with shamixanthone (3) and tajixanthone hydrate (4). The stereostructures of 1 and 2 were elucidated by spectroscopic and molecular modeling methods. The absolute configuration at the stereogenic centers of 1 was established according to CD measurements. In the case of 2, however, the absolute configuration at C-20 and C-25 was designated as S and R, respectively, by Mosher ester methodology. Thereafter, the configuration at C-14 and C-15 of 2 was established as S and S, respectively by comparing the optical rotation and 1H–1H coupling constant experimental values with those obtained through molecular modeling calculations at DFT B3LYP/DGDZVP level of theory for diasteroisomers 2a–2d. The activation of the calmodulin-sensitive cAMP phosphodiesterase (PDE1) was inhibited in the presence of 1–4 in a concentration-dependent manner. The effect of compounds 2 (IC50 = 5.54 μM) and 4 (IC50 = 5.62 μM) was comparable with that of chlorpromazine (CPZ; IC50 = 7.26 μM), a well known CaM inhibitor used as a positive control. The inhibition mechanism of both compounds was competitive with respect to CaM according to a kinetic study. A docking analysis with 2 and 4 using the AutoDock 4.0 program revealed that they interacted with CaM in the same pocket as trifluoropiperazine (TFP). |
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