Selective protein modification by the hydroperoxide intermediate in a photoprotein,aequorin期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

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Selective protein modification by the hydroperoxide intermediate in a photoprotein,aequorin

Authors:	Issei Doi Masaki Kuse Toshio Nishikawa Minoru Isobe

Institution:	1. Laboratory of Organic Chemistry, Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8602, Japan;2. Chemical Instrument Division, Research Center for Materials Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8602, Japan;3. Institute for Advanced Research, Nagoya University, Nagoya 464-8601, Japan;4. Department of Chemistry, National Tsing Hua University, Hsinchu, Taiwan

Abstract:	During the course of protein modification program, we employed a recombinant aequorin, the apo-protein reconstituted with coelenterazine, and found out that the photolytic hyperperoxide modified three –S–SCH₂CHOHCHOHCH₂SH groups to –S–SCH₂CHOHCHCH–SO)H or –S–SCH₂CHOHCHCH–S(O)OH of terminal DTT connected to cysteine residues of the C¹⁴⁵, C¹⁵² and C¹⁸⁰, which turned out to locate near the chromophore.

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