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Karyotyping by PFGE of clinical isolates of Sporothrix schenckii |
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| Authors: | Takeshi Tateishi Somay Yamagata Murayama Fujio Otsuka Hideyo Yamaguchi |
| Institution: | Department of Dermatology, University of Tsukuba, School of Medicine 1-1-1 Tennōdai, Tsukuba-city, Ibaraki 305, Japan; Department of Microbiology and Immunology, Teikyo University School of Medicine 2-11-1 Kaga, Itabashi-ku, Tokyo 173, Japan; Teikyo University Research Center for Medical Mycology 359 Otsuka, Hachioji, Tokyo 192-03, Japan |
| Abstract: | Abstract From October 1991 to December 1992 we had eight patients with sporotrichosis at Tsukuba University Hospital in Japan. With 8 strains isolated from these patients, PFGE (pulsed-field gel electrophoresis) analyses were carried out to examine whether the karyotype of S. schenckii is distinguished by our method and whether this molecular approach is a useful means of biotyping of S. schenckii strains. Chromosomes were separated by contour-clamped homogeneous electric field (CHEF) gel electrophoresis. The strains had six to eight chromosomes and a total genome size was approx. 28 Mbp. Although these karyotypes of all the isolates looked closely similar to each other, they were grouped into three types. |
| Keywords: | Sporothrix schenckii: Sporotrichosis Pulsed-field gel electrophoresis Karyotype |