Molecular analysis of Helicobacter pylori populations in antral biopsies from individual patients using randomly amplified polymorphic DNA (RAPD) fingerprinting |
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| Authors: | David G Marshall rew Chua PW Napoleon Keeling Derek J Sullivan David C Coleman Cyril J Smyth |
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| Institution: | University of Dublin, The Moyne Institute, Department of Microbiology, Trinity College, Dublin 2, Ireland;University of Dublin, Medical School, Departments of Clinical Medicine and Gastroenterology, Trinity College Dublin, St. James's Hospital, Dublin 8, Ireland;University of Dublin, School of Dental Science, Department of Oral Medicine and Pathology, Trinity College, Dublin 2, Ireland |
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| Abstract: | Abstract In the present study, randomly amplified polymorphic DNA (RAPD) fingerprinting has been used to analyse multiple single colony isolates of Helicobacter pylori from antral biopsies in an attempt to ascertain whether or not multiple strains are present in individual patients using single biopsy samples. The RAPD fingerprints derived from single colonies obtained from the same biopsy specimen were in all cases indistinguishable. The previously noted heterogeneity between H. pylori strains from different individuals was confirmed. RAPD fingerprinting, combined with a simple method of template preparation, was shown to be an excellent method for H. pylori strain differentiation. The results of this study indicate that the H. pylori population is homogeneous in individual patients at a single gastric site. |
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| Keywords: | Helicobacter pylori Randomly amplified polymorphic DNA Strain differentiation Fingerprinting Bacterial typing |
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