Duplication of insertion element IS50 associated with Tn5 transposition in Azospirillum brasilense |
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Authors: | Tripathi A K Tripathi R Ganguli A Bazzicalupo M |
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Institution: | Laboratory of Bacterial Genetics, School of Biotechnology, Faculty of Science, Banaras Hindu University, Varanasi, India. tripathianil@hotmail.com |
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Abstract: | The characterization of a DNA fragment with a Tn5 insertion in a regulatory nif gene of Azospirillum brasilense is reported. Restriction endonuclease mapping, Southern hybridization with a Tn5 probe, and nucleotide sequencing revealed that IS50 had duplicated in Tn5. The duplication of an IS50 element suggests the occurrence of a replicative mechanism of transposition. A strategy, based on the bacterial ability of homologous recombination that was used to precisely eliminate Tn5 along with the duplicated IS50 element, is presented. |
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