Identification of low-dye-binding (ldb) mutants of Saccharomyces cerevisiae |
| |
Authors: | Corbacho Isaac Olivero Isabel Hernández Luis M |
| |
Institution: | Department of Microbiology, University of Extremadura, 06071 Badajoz, Spain. |
| |
Abstract: | We have completed the identification of Saccharomyces cerevisiae genes that are defective in previously isolated ldb (low-dye-binding) mutants. This was done by complementation of the mutant's phenotype with DNA fragments from a genomic library and by running standard tests of allelism with single-gene deletion mutants of similar phenotype. The results were as follows: LDB2 is allelic to ERD1; LDB4 to SPC72; LDB5 to RLR1; LDB6 to GON7/YJL184W; LDB7 to YBL006C; LDB9 to ELM1; LDB10 to CWH36; LDB11 to COG1; LDB12 to OCH1; LDB13 to VAN1; LDB14 to BUD32; and LDB15 to PHO85. Since the precise function of some of the genes is not known, these data may contribute to the functional characterization of the S. cerevisiae genome. |
| |
Keywords: | Saccharomyces cerevisiae Glycosylation Deletion mutant ldb mutant Oligosaccharide phosphorylation |
本文献已被 PubMed 等数据库收录! |