中国近海银鲳线粒体COⅠ基因序列变异分析

对采自黄海、东海和南海的7个银鲳群体的线粒体COⅠ基因序列变异进行分析,研究银鲳的遗传多样性、遗传结构和群体历史动态。在所分析的111个个体中检测到16个单倍型。7个群体呈现出高的单倍型多样性（h=0.564～0.688） 和低的核苷酸多样性（π=0.001～0.003）。单倍型遗传学关系、两两群体间的F_ST值和分子方差分析均表明中国近海7个银鲳群体间的遗传分化不显著。中性检验和核苷酸不配对分析均表明中国近海银鲳经历了晚更新世的群体扩张事件,扩张时间约为6.0×10⁴～1.04×10⁵ 年前。研究结果表明,银鲳的卵和幼体具有较强的扩散能力、中国近海的海洋环流以及近期的群体扩张可能是造成中国近海银鲳群体在线粒体COⅠ基因序列上存在较高的遗传同质性的原因。

MITOCHONDRIAL COⅠ SEQUANCE VARIATION OF SILVER POMFRET (PAMPUS ARGENTUS) FROM CHINESE COASTAL WATERS

To evaluate the population genetic diversity,genetic structure and demographic history of Silver pomfret(Pampus argenteus),we sequenced the mtDNA COⅠ gene(591 bp) in 111 individuals of 7 localities sampled from the Yellow Sea,East China Sea and South China Sea.A total of 16 haplotypes were defined among 111 individuals.High haplotype diversity(0.564-0.688) and low nucleotide diversity(0.001-0.003) were detected within each population.T he genetic relationships of haplotypes,pairwise FST comparisons and hierarchical molecular variance analysis(AM OVA) showed no significantgenetic differences between populations in P.argenteus.T he neutrality tests and mismatch distribution analysis revealed that P.argenteus had undergone population expansion in the late Pleistocene.And the population expansion occurred approximately at 6.0 × 104-1.04 × 105 years ago.T he results of this study indicated that P.argenteus constituted a panmictic mtDNA COⅠ gene pool from the Yellow Sea,East China Sea and South China Sea.Factors such as dispersal capacity,ocean currents and insufficient evolution time could be responsible for the lack of population genetic differentiation in P.argenteus.