| 五种水螅基因组DNA的RAPD多态性研究 |
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| 引用本文： | 范学铭,肖玮,张彦帅.五种水螅基因组DNA的RAPD多态性研究[J].动物分类学报,2005,30(1):205-208. |
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| 作者姓名： | 范学铭 肖玮 张彦帅 |
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| 作者单位： | 哈尔滨师范大学生命与环境科学学院生物学系,哈尔滨,150080 |
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| 基金项目： | 黑龙江省自然科学基金，黑龙江省教育厅自然科学基金 |
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| 摘 要： | 利用RAPD技术对水螅科2属5种水螅进行了基因组DNA多态性研究.80个随机引物筛选出18个引物,共扩增出303个清晰稳定的DNA片段,分子量在0.3～3.0kb之间.以UPGMA聚类法构建树状图,阐明水螅属种的分类关系:大庆水螅Hydra daqingensis、北京水螅H.beijingensis和强壮水螅H.robusta 3种分成两组并聚在一起;不同属的漠河水螅H.mohensis和绿水螅Chlorohydra sp.聚为一支;由此2属5种水螅分为两个支系.
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| 关 键 词： | 大庆水螅 北京水螅 强壮水螅 漠河水螅 绿水螅 |
GENETIC DNA DIVERSITY OF FIVE SPECIES OF HYDRAS |
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| FAN Xue-Ming,XIAO Wei,ZHANG Yan-Shuai.GENETIC DNA DIVERSITY OF FIVE SPECIES OF HYDRAS[J].Acta Zootaxonomica Sinica,2005,30(1):205-208. |
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| Authors: | FAN Xue-Ming XIAO Wei ZHANG Yan-Shuai |
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| Institution: | FAN Xue-Ming,XIAO Wei,ZHANG Yan-Shuai Department of Biology,College of Life and Environment Science,Harbin Normal University,Harbin 150080,China |
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| Abstract: | RAPD (random amplified polymorphic DNA) technique was applied to assess the genetic diversity among five species belonging to two genera of Hydraridae. Eighteen primers were selected from 80 random primers and yielded a total of 303 clear bands ranging from 0.3-3.0?kb. The classification relationships of hydras were clarified using UPGMA. Individuals of the same species clustered respectively first. Hydra daqingensis and H. beijingensis showed close relationship with H. robusta, H. mohensis and Chlorohydra sp. belonging to different genera showed close relationship, so five species of hydras could be divided into two groups. |
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| Keywords: | RAPD |
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