大连滑刃线虫新种(线虫门,滑刃科)记述(英文)

新种大连滑刃线虫.Aphelenchoides dalianensis sp.nov.采自中国辽宁省大连市老铁山的枯死黑松.新种的鉴别特征为:体较短(雌虫:571.5～658.0 μm;雄虫:436.8～520.0μm),口针纤细(雌虫:10.0～12.7 μm;雄虫:9.2～11.8μm)具有基部球,侧线4条.雌虫阴门位于虫体60%～75%处,尾型特殊,具蜗牛触角状分叉的尾尖突;雄虫尾部向腹面弯曲成拐杖形,有1简单尾尖突,交合刺小(10.0～12.9μm),乳突3对,无交合伞.新种的近似种是大核滑刃线虫A.macronucleatus,主要区别在于大核滑刃线虫的雌虫仅具一简单尾尖突,雄虫加热杀死后呈"L"形,而非新种的"J"形.应用限制性酶切图谱(PCR-ITS-RFLP)的方法以及DNA测序为新种提供了分子生物学的证据.

APHELENCHOIDES DALIANENSIS SP. NOV. (NEMATODA,APHELENCHOIDIDAE) FROM PINUS THUNBERGII FROM CHINA

A new species of nematode, Aphelenchoides dalianensis sp. nov. was extracted grom a wilt black pine (Pinus thunbergii) from Laotieshan (38.8°N, 121.2°E), Dalian City, China. A. dalianensis was cbaracterized by its length (females: 571.5-658.0 μm; males: 436.8-520.0 μm); slender stylets (females: 10.0-12.7 μm; males: 9.2-11.8 μm) with basal knobs; 4 lateral lines. Female vulva post median, at 60%-75% of the body length; female tail with a special mucronate struaure which had a divarication, forming an antennae-like terminus; male tail strongly hooked ventrally to from the charactenstic 'walking-stick' form, tapenng to a single terminus; small spicule (10.0-12.9 μn); 3 pairs of caudal papillae and bursa absent. A. macronucleatus resembles the new species, especially in male spicule, but differs from female tail terminus and the shape of male after heat-killed. The PCR-ITS-RFLP pattern and its rDNA sequence also provided further evidence that this isolate is 1 new species. Aphelenchoides dalianensis sp. nov. was named by the name of city, Dalian, whae it was found.