Localization and Identification of Populations of Phosphatase-Active Bacterial Cells Associated with Activated Sludge Flocs |
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Authors: | F Van Ommen Kloeke GG Geesey |
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Institution: | (1) Department of Microbiology and Center for Biofilm Engineering, Montana State University, Bozeman, MT 59717-3980, USA, US |
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Abstract: | Abstract
The majority of phosphatase (PO4ase) activity detected in fresh aerobic activated sludge from a municipal wastewater treatment plant was associated with suspended
floc material. PO4ase activity appeared to be localized in discrete bacteria-containing areas of the floc matrix based on the distribution of
nucleic acid–stained cells and precipitated fluorescent crystals produced as a result of reaction of the enzyme(s) with the
artificial substrate ELF™-PO4. Of the total floc-associated bacterial cells that stained positive with the nucleic acid–binding fluorochrome acridine orange
(AO), 8.8 ± 1.2% displayed PO4ase activity based on the proximity of AO-stained cells to precipitated ELF crystals. Using a 16S rRNA oligonucleotide probe
specific for the cytophaga–flavobacteria group, it was determined that 17–20% of the floc-associated bacteria that probed
positive also displayed PO4ase activity. Furthermore, 35–45% of the ELF fluorescence was associated with bacterial cells that probed positive for the
cytophaga–flavobacteria group. The results suggest that the cytophaga–flavobacteria, as a group, is important in mediating
the liberation of inorganic orthophosphate (Pi) from phosphomonoesters of detrital organic phosphate (organic-P) in the aerobic activated sludge process of wastewater treatment.
Received: 17 March 1999; Accepted: 9 June 1999 |
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