Monitoring of Biodegradative Pseudomonas putida Strains in Aquatic Environments Using Molecular Techniques |
| |
Authors: | H Wand T Laht M Peters PM Becker U Stottmeister A Heinaru |
| |
Institution: | (1) University of Leipzig, Faculty of Biological Sciences, Pharmacy and Psychology, Department of Biotechnology, Permoserstrasse 15, 04318 Leipzig, Germany, DE;(2) University of Tartu, Institute of Molecular and Cell Biology, Riia 23, EE2400 Tartu, Estonia, EE;(3) Center for Environmental Research (UFZ) Leipzig-Halle Ltd., Department of Remediation Research, Permoserstrasse 15, 04318 Leipzig, Germany, DE |
| |
Abstract: | Abstract
Monitoring strategies were developed to track non–genetically engineered Pseudomonas putida strains in the open environment. The strain E1 was used for four years for the biodegradation of phenolic compounds in industrial
wastewater in P?lva, Estonia. In this study we used the strain E2 which is a non-carbenicillin-resistant variant of the strain
E1. Both strains have a deletion of approximately 34 kb in the TOL plasmid pWW0 which served as a basis for discrimination
from indigenous bacteria by molecular analyses. Other targets used for PCR and DNA hybridization were the xylE gene and a sequence located in the left-handed region of to the transposon Tn4652. In laboratory tests we demonstrated that two cells inoculated into 20 ml of river water could be detected against a background
of more than 107 colony forming units (CFUs) by a combination of growth on selective media and molecular analysis. Using the same combination
of methods in a deliberate release experiment, detection of the released strain was possible only to 32 h after release. It
is assumed that the released strains did not survive in the aquatic ecosystem, mainly due to the high dilution rate. The combination
of cultivation on selective media and molecular analyses proved useful for tracking Pseudomonas putida strain E2 in an aquatic environment.
Received: 29 March 1996; Accepted: 1 April 1996 |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|