Distinctive Phyllosphere Bacterial Communities in Tropical Trees |
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Authors: | Mincheol?Kim Dharmesh?Singh Ang?Lai-Hoe Rusea?Go Raha?Abdul Rahim Ainuddin?AN Jongsik?Chun Email author" target="_blank">Jonathan?M?AdamsEmail author |
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Institution: | (1) School of Biological Sciences, Seoul National University, 599 Gwanak-ro, Gwanak-gu, Seoul, 151-747, Republic of Korea;(2) Forest Biotechnology, Forest Research Institute of Malaysia, Kepong, Malaysia;(3) INTROP, Universiti Putra Malaysia, Serdang, Selangor, 43400, Malaysia;(4) Institute of Biotechnology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, Selangor, 43400, Malaysia;(5) Department of Biology, Universiti Putra Malaysia, Serdang, Selangor, 43400, Malaysia; |
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Abstract: | Recent work has suggested that in temperate and subtropical trees, leaf surface bacterial communities are distinctive to each
individual tree species and dominated by Alpha- and Gammaproteobacteria. In order to understand how general this pattern is,
we studied the phyllosphere bacterial community on leaves of six species of tropical trees at a rainforest arboretum in Malaysia.
This represents the first detailed study of ‘true’ tropical lowland tree phyllosphere communities. Leaf surface DNA was extracted
and pyrosequenced targeting the V1–V3 region of 16S rRNA gene. As was previously found in temperate and subtropical trees,
each tree species had a distinctive bacterial community on its leaves, clustering separately from other tree species in an
ordination analysis. Bacterial communities in the phyllosphere were unique to plant leaves in that very few operational taxonomic
units (0.5%) co-occurred in the surrounding soil environment. A novel and distinctive aspect of tropical phyllosphere communities
is that Acidobacteria were one of the most abundant phyla across all samples (on average, 17%), a pattern not previously recognized.
Sequences belonging to Acidobacteria were classified into subgroups 1–6 among known 24 subdivisions, and subgroup 1 (84%)
was the most abundant group, followed by subgroup 3 (15%). The high abundance of Acidobacteria on leaves of tropical trees
indicates that there is a strong relationship between host plants and Acidobacteria in tropical rain forest, which needs to
be investigated further. The similarity of phyllosphere bacterial communities amongst the tree species sampled shows a significant
tendency to follow host plant phylogeny, with more similar communities on more closely related hosts. |
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