The Use of Carbon Substrate Utilization Patterns in Environmental and Ecological Microbiology |
| |
Authors: | A Konopka L Oliver RF Turco Jr |
| |
Institution: | (1) Department of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA, US;(2) Department of Agronomy, Purdue University, West Lafayette, IN 47907, USA, US |
| |
Abstract: | Abstract
Carbon substrate utilization patterns have found increasing use in environmental and ecological microbiology over the past
five years. Ninety six-well microtiter plates containing various carbon substrates permit these patterns to be determined
quickly, economically, and effectively. The use of these patterns to characterize and differentiate strains isolated from
the environment has been very effective in providing information on the culturable fraction of the microbial community. Another
approach involves the direct inoculation of natural samples into these microtiter plates; this approach has several fundamental
problems. The inoculation of low cell densities into the wells means that the technique is a culture-based method in which
the biases of enrichment culture may render the results unrepresentative of the native microbiota. The physiological state
of the inoculated microbes may affect the kinetics and pattern of substrate utilization. As a measure of the functional diversity
of microbial communities, this approach suffers because the tested substrates do not accurately represent the types of substrates
present in ecosystems, and the metabolic redundancy of species implies that changes in the response may only crudely represent
the actual microbial population dynamics. Therefore, although this approach can be used to determine whether environmental
samples differ in their response patterns, it is unclear how it can be used to provide fundamental information on questions
of microbial diversity.
Received: 14 March 1997; Accepted: 2 June 1997 |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|