Induction of DNA polymerase in mouse L cells

Two molecular species of DNA polymerase are found in mouse L cells. This study is concerned with the variation of these two species of enzyme with the rate of cell growth and DNA synthesis. The 3.4 S DNA polymerase, found in both nuclear and cytoplasmic fractions of mouse L cells, remains relatively constant during different stages of the growth curve. The heterogeneous 6 to 8 S DNA polymerase, found only in the cytoplasmic fractions, varies about 5 to 12-fold in correlation with DNA synthesis, as measured by ³H]thymidine incorporation.