Abortive bacteriophage T4 head assembly in mutants of Escherichia coli |
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Authors: | Anna Coppo Andrea Manzi John F Pulitzer Hideo Takahashi |
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Institution: | International Institute of Genetics and Biophysics C.N.R., Naples, Italy |
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Abstract: | We describe two mutants (tabB-212 and tabB-127) of Escherichia coli K12 in which T-even phage production is temperature-sensitive. Both mutants are linked to purA and may identify a single new bacterial gene tabB. The uninfected bacterium is indistinguishable from wild type at both 30 °C and 42.4 °C. Sodium dodecyl sulphate—polyacrylamide gel electrophoresis of labelled extracts of tabB mutants infected by T4 wild-type phage shows that the modification of viral head precursors (Laemmli, 1970) does not occur, indicating that capsid formation is blocked. The effect is reversible with at least one of the tabB mutants: a shift to 30 °C leads to the cleavage of a significant fraction of precursors synthesized at 42.4 °C.Two classes of T4 mutants are described: one (comB) which grows on tabB even at 42.4 °C, the other (kB) which fails to grow on tabB even at the permissive temperature. Both mutants map in T4 gene 31, suggesting an interaction between gene 31 and tabB products.Since gene 31 mutants lead to the random aggregation of head precursors (Laemmli, 1970), we argue that a host product is involved in the ordered polymerization of T4 proteins into capsids or capsid-related structures. |
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Keywords: | internal protein Cam chloramphenicol |
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