Structure of tomato bushy stunt virus: V. Coat protein sequence determination and its structural implications |
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Authors: | P Hopper SC Harrison RT Sauer |
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Institution: | 1. Department of Biology Massachusetts Institute of Technology, Cambridge, MA 02139, U.S.A.;2. Department of Biochemistry and Molecular Biology Harvard University, 7 Divinity Avenue, Cambridge, MA 02138, U.S.A. |
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Abstract: | We report the chemically determined sequence of most of the polypeptide chain of the coat protein of tomato bushy stunt virus. Peptide locations have been determined by comparison with the high-resolution electron density map from X-ray crystallographic analysis as well as by conventional chemical overlaps. Three small gaps remain in the 387-residue sequence. Positively charged side-chains are concentrated in the N-terminal part of the polypeptide (the R domain) as well as on inward-facing surfaces of the S domain. There is homology of S-domain sequences with structurally corresponding residues in southern bean mosaic virus. |
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Keywords: | TBSV tomato bushy stunt virus SBMV southern bean mosaic virus TCV turnip crinkle virus STNV satellite of tobacco necrosis virus u v ultraviolet light TPCK-trypsin |
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