Demonstration of a highly exposed region in Escherichia coli 5 s RNA by partial hydrolysis with ribonuclease IV and sheep kidney nuclease

Partial digests of ³²P-labelled 5 s RNA with Escherichia coli RNase IV and with sheep kidney nuclease were fractionated by two-dimensional polyacrylamide gel electrophoresis. The nucleotide sequences of the various fractions were determined by fingerprinting analysis. The results show the existence of a single-stranded loop around position G₄₁ of the 5 s RNA molecule.