Preliminary crystallographic studies of the amino terminal half of human lactoferrin in its iron-saturated and iron-free forms. |
| |
Authors: | C L Day G E Norris B F Anderson J W Tweedie E N Baker |
| |
Institution: | Department of Chemistry and Biochemistry, Massey University, Palmerston North, New Zealand. |
| |
Abstract: | The amino terminal half of human lactoferrin (LfN) produced from transfected baby hamster kidney cells has been crystallized in its iron-saturated and iron-free forms. The crystals of glycosylated LfN and deglycosylated LfN are monoclinic, space group C2, with cell dimensions a = 133.0 A, b = 58.3 A, c = 58.3 A, alpha = 90.0 degrees, beta = 114.7 degrees, gamma = 90.0 degrees, and one molecule per asymmetric unit. Crystals of apo LfN have also been prepared using deglycosylated protein. These crystals are tetragonal, space group P4(1)2(1)2 (or P4(3)2(1)2), with cell dimensions of a = b = 58.4 A and c = 217.2 A and one molecule per asymmetric unit. Both the iron-saturated and the iron-free crystals are suitable for high resolution X-ray analysis. |
| |
Keywords: | |
|
|