Crystallization of complexes of EcoRV endonuclease with cognate and non-cognate DNA fragments |
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| Authors: | F K Winkler A D'Arcy H Bl?cker R Frank J H van Boom |
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| Institution: | European Molecular Biology Laboratory, Heidelberg, Germany. |
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| Abstract: | Complexes of the type II restriction endonuclease EcoRV with a variety of short, selfcomplementary deoxyoligonucleotides have been crystallized. The best crystals diffract to about 2.7 A resolution and consist of 1:1 complexes between endonuclease dimers and duplexes of the cognate decamer GGGATATCCC containing the hexameric RV recognition sequence GATATC. Crystals with the non-cognate DNA octamer duplexes CGAGCTCG and CGAATTCG diffract to 3.0 and 3.5 A resolution, respectively, and contain two DNA duplexes per enzyme dimer. |
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