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Sites of dnaA protein-binding in the replication origin of the Escherichia coli K-12 chromosome
Authors:M Matsui  A Oka  M Takanami  S Yasuda  Y Hirota
Institution:Institute for Chemical Research Kyoto University Uji, Kyoto 611, Japan;National Institute of Genetics Mishima 411, Japan
Abstract:On the basis of the observation that dnaA protein binds preferentially to DNA fragments carrying the Escherichia coli chromosomal replication origin (oriC), the binding sites were investigated by DNase I footprinting. As a result, three strong binding sites were identified in the minimal oriC sequence. The respective binding sites were 16 to 17 base-pairs long, and contained a common sequence (5') T-G-T-G-(G/T)-A-T-A-A-C (3') in the middle, although their polarities were not the same. Since mutants defective in function for autonomous replication have been isolated in the corresponding positions of the common sequence at each binding site, dnaA protein-binding at these sites seems to be significant for replication initiation.
Keywords:Author to whom correspondence should be addressed  
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