中国林蛙乳酸脱氢酶多基因系统及基因间连锁关系的研究

（１）用聚丙烯酰胺凝胶电泳对山西产中国林蛙４个地理群的３３３只林蛙进行了分析，结果表明：中国林蛙的ＬＤＨ由ＬＤＨ－Ａ，ＬＤＨ－Ｂ和ＬＤＨ－Ｃ３个基因决定。ＬＤＨ－Ａ是单态座位，ＬＤＨ－Ｂ和ＬＤＨ－Ｃ均为多态座位，每个多态座位均有两个等位基因。ＬＤＨ－Ｂ与ＬＤＨ－Ｃ呈紧密连锁关系。认为ＬＤＨ－Ｃ是ＬＤＨ－Ｂ的重复产物。（２）热稳定性、尿素处理稳定性及组织特异性研究表明：ＬＤＨ对温度和尿素处理稳定性顺序为Ａ４＞Ｂ’４＞Ｂ４＞Ｃ４。Ａ４在骨骼肌和肝脏等组织中活力最大，Ｂ４在心肌和卵巢中活力最强，ＬＤＨ－Ｃ主要在眼球和卵巢中表达。（３）Ｌｄｈ－ｂ和Ｌｄｈ－ｂ＇在不同地理群间呈差异分布，随着纬度的增高，Ｌｄｈ－ｂ在种群中的频率增大。

Studies on the Multiple Gene System and Gene Linkage of Lactate Dehydrogenase in Rana chensinensis

With polyacrylamide gel electrophoresis,LDH isozymes of 333 individuals of Rana chensinensis were analyzed,the frogs were sampled from four geographic population in Shanxi province, P.R. China,The results indicated that the LDH isozymes of Rana chensinensis are determined by three genes,they are LDH-A,LDH-B,LDH-C, LDH-A is a monomorphic locus,both LDH-B and LDH-C are polymopohic loci and each locus has two alleles, respectively.LDH-B links with LDH-C closely,it is assumed that LDH-C was produced by duplication of LDH-B.(2)Studies on the thermalstability,urea sensitivity and specific distribution in tissuse showed that the order of LDH isozymes stability for heat and urea treatment is A4>B4>C4. A4 is abundent in skeleten muscle and B4 predominent in heart muscle and ovary. LDH-C expresses specifically in eye and ovary.(3) Ldh-b and Ldh-b'distribute differently in different geographic populations, with the increase of latitude,the frerquence of Ldh-b becomes greater in population.